At its peak, 62,000 people watched a stream of the game, which was hosted by esports personality Ibai.
"As a sports athlete, you don't spend more than three or four hours a day doing that so you have a lot of off time and a lot of that off time is spent doing video games," Carlos Rodriguez, founder and owner of G2 Esports team which hosted the match, told CNN Sport.
"These two worlds collide a lot ... these athletes both play and watch a lot of esports and video game related stuff.
"It's only natural for them. They know who are (esports') biggest stars, who are the best players, and of course they want to play with them."
Rodriguez says that the crossover between real and virtual sports is beneficial to both communities in terms of bringing in new fans, adding that the streaming numbers of the Seville derby were two or three times greater than usual.
"If an athlete decides to stream you will undoubtedly get good numbers because these guys are really large and the gaming community really loves it when they get embraced by known personalities," said Rodriguez.
"When you go out and decide to stream or play a video game in front of everybody, people will be wanting to watch it definitely."
The virtual Seville derby ended 6-5 in Betis' favor after Iglesias scored the winning goal -- with himself.
Ibai, a content creator for G2, also posted a video where he explained to Reguilon, Iglesias, Real Madrid goalkeeper Thibaut Courtois and Borussia Dortmund's Achraf Hakimi how to play League of Legends.
English club Leyton Orient is setting up a knockout tournament on FIFA, with teams from Australia, Denmark and the Netherlands -- as well as from England, Scotland and Ireland -- all agreeing to take part.
The idea, which started after football club's Twitter accounts played each other at Connect 4, will see sides put forward a representative to play as their club on FIFA.
The initial competition -- dubbed FIFA Quaran-Team, a play on the game mode FIFA Ultimate Team -- would have featured 64 teams but it has swelled to 128 after a high level of interest on social media.
It seems fans can breathe a slight sigh of relief; maybe there will be some sort of football to watch after all.